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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

6-8 weeks 2 vials 6 weeks: 42 doses needed

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Convenience & Compliance: Ease of administration influences real-world effectiveness

Its most significant and well-understood roles are: Collagen synthesis: Vitamin C is an essential co-factor for the enzymes prolyl hydroxylase and lysyl hydroxylase, which are required to stabilise the triple-helix structure of collagen fibres

Journal of the Association of Physicians of India